Beckford: Ankle injury
Leeds' leading goalscorer returned to the starting line-up for the first-leg defeat at Elland Road but was replaced just before the hour mark.
Beckford had missed the previous four matches due to an ankle problem first sustained in the win at Leyton Orient early last month.
Tresor Kandol and Anthony Elding are on stand-by if Beckford fails to make it.
Manager Gary McAllister, who has no other new injury problems, has confirmed veteran midfielder Alan Thompson will be included in a 20-man squad.
However, on-loan Leicester defender Alan Sheehan remains sidelined through suspension, so Rui Marques and Darren Kenton will be hoping to play a bigger part as Leeds bid to overturn a 2-1 deficit to reach the Wembley final.